The Role of TLR4 on PGC-1α-Mediated Oxidative Stress in Tubular Cell in Diabetic Kidney Disease

Figure 7

Overexpression of PGC-1α diminished HG-induced caspase-3 expression in HK-2 cells. HK-2 cells were transfected with pcDNA4 myc PGC-1α or pcDNA4 myc (empty vector) for 24 h. (a) A1: The mRNA level of PGC-1α. A2: The mRNA level of cleaved caspase-3. (b) B1-B2: The protein expression of PGC-1α and cleaved caspase-3 were examined by Western blotting. (c) The protein expression of NF-κB p65 and phospho-NF-κB p65 with HG for 2 h. (d) D1-D2: The protein expression of phospho-NF-κB p65 with HG for 24 h. Each assay was representative of three independent experiments. Data were expressed as ; , , and .

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