Impaired Mitophagy of Nucleated Erythroid Cells Leads to Anemia in Patients with Myelodysplastic Syndromes

Figure 4

The mitochondrial dysfunction in GlycoA⁺ NRBC measured by FCM. (a) The amount of mitochondria (MTDR levels) in GlycoA⁺ NRBC in high-risk MDS was higher than that in controls and low-risk MDS. (b) The ΔΨ_m in GlycoA⁺ NRBC in high-risk MDS was lower than that in controls and low-risk MDS. (c) The level of ROS in GlycoA⁺ NRBC in high-risk MDS was higher than that in controls and low-risk MDS. (d) The expression of the mitochondrial outer membrane protein TOM20 in GlycoA⁺ NRBC tested by Western blot. The intensity ratio of TMO20/β-actin tested by Western blot in the three groups (, ).

(a)

(b)

(c)

(d)