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Oxidative Medicine and Cellular Longevity
Volume 2018 (2018), Article ID 6354972, 12 pages
Research Article

36H: A Novel Potent Inhibitor for Antimelanogenesis

1Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, Kaohsiung 807, Taiwan
2School of Medical and Health Sciences, Fooyin University, Kaohsiung 831, Taiwan
3Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung 811, Taiwan
4Department of Biological Science and Technology, Meiho University, Pingtung 912, Taiwan
5Division of Bioengineering, Incheon National University, Incheon, Republic of Korea
6University of British Columbia, Department of Integrated Sciences for Physiology and Behavioural Neuroscience, Vancouver, BC, Canada
7Department of Neuroscience, Washington State University, Pullman, WA, USA
8Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan
9Department of Biotechnology, Asia University, Taichung 413, Taiwan
10Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan
11Center for Stem Cell Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan
12Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan

Correspondence should be addressed to Yueh-Hsiung Kuo and Hui-Min David Wang

Received 13 June 2017; Revised 15 October 2017; Accepted 5 November 2017; Published 4 February 2018

Academic Editor: Fatma M. El-Demerdash

Copyright © 2018 Li-Ching Lin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


N-Hydroxycinnamoylphenalkylamides (36H) exhibited both antioxidation and antityrosinase abilities. The compound was studied for its antioxidative properties, using a 1,1-diphenyl-2-picrylhydrazul- (DPPH-) scavenging test, a ferric ion-reducing antioxidant power assay (FRAP) assessment, and a metal-chelating power assay. The results showed that 36H had antioxidative capabilities in the DPPH-scavenging and ferric-reducing power examinations but the chelating power assay did not demonstrate antioxidative capability. 36H was also measured for tyrosinase inhibitory activity applying various species platforms, including in vitro mushroom, B16F10 mouse melanoma, and human melanocyte cells. In terms of in vitro mushroom tyrosinase suppression, 36H restrained the melanogenesis processes. It is assumed that 36H blocked the tyrosinase active site as a competitive inhibitor for mushroom tyrosinase, hence not decreasing the human normal melanocyte cellular viability. A quantitative real-time polymerase chain reaction (qRT-PCR) and western blot discovered that 36H downregulated melanogenesis-related RNA and proteins, including pigment production (MITF, tyrosinase, TRP-1, and TRP-2), melanosome maturation (Rab27a), and melanosome transportation (Myo5a, MLPH and Mreg). Overall, 36H displayed the biofunctions of antioxidation and melanin suppression, so there was a possibility for its application as a food additive or a skin-whitening agent.