Oxidative Stress in Cardiac Tissue of Patients Undergoing Coronary Artery Bypass Graft Surgery: The Effects of Overweight and Obesity

Figure 3

Expression of antioxidant proteins in cardiac tissue. (a) Expression of mitochondrial superoxide dismutase (Mn-SOD) and the cytosolic isoform (Cu,Zn-SOD) was determined by western blot analysis. The values are shown as percentage of control (normal BMI). Original blots are shown below the densitometric quantification. The signals of the SODs were normalized to the loading control—beta-actin. (b) Expression of the mitochondrial aldehyde dehydrogenase (ALDH-2) by western blot analysis. Original blots are shown below the densitometric quantification. The signal of the ALDH-2 was normalized to the loading control—beta-actin. ALDH-2 activity was determined by Monal-62 as a substrate and HPLC-based quantification. (c) Correlations between cardiac oxidative stress (2-HE and ethidium) and ALDH-2 activity (Monal-62) are shown. Ethidium (, ); 2-HE (, ). (d) Expression of heme oxygenase-1 (HO-1) by western blot analysis. Original blots are shown below the densitometric quantification. The signal of HO-1 was normalized to the loading control—beta-actin. The correlation between BMI and HO-1 is shown (, ). Control: BMI < 25 kg/m²; overweight: BMI 25–29 kg/m²; obese: BMI > 30 kg/m². All data are expressed as mean ± SEM from (a), 61 (expression)/33 (activity) (b), and 61 (c) independent measurements/patients. vs. Ctr. group; vs. Ctr. group.

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