Protocatechuic Acid Attenuates Trabecular Bone Loss in Ovariectomized Mice

Figure 6

Effects of PCA on mRNA expression level of TRAF6 and NFATc1. OVX mice received vehicle, PCA (10 and 20 mg/kg b.w./day), or E₂ for 12 weeks. At the end of the experiment, bone marrow cells were isolated, and the mRNA expression levels of cathepsin K (a) and calcitonin receptor (b) were measured by real-time RT-PCR. β-Actin was used as a loading control for RT-PCR. The results are expressed as a mean ± SD (each group, ). between the sham and OVX-alone group. among OVX groups with or without interventions. among OVX groups with interventions. b.w.: body weight; E₂: 17β-estradiol; OVX; ovariectomized; PCA: protocatechuic acid; TRAF6: TNF receptor-associated factor-6; NFATc1: nuclear factor of activated T cell cytoplasmic 1.

(a)

(b)