Research Article

The Causal Role of Mitochondrial Dynamics in Regulating Insulin Resistance in Diabetes: Link through Mitochondrial Reactive Oxygen Species

Figure 4

Both Drp1 and Fis1 deteriorate cellular insulin resistance. Cybrid B4 was starved of FBS for 16 h, transfected with plasmid/siRNA for 24/48 h, and then treated with 0, 0.1, or 1 μM insulin for 1 h. (a, d) p-IRS1-1(Y896), IRS-1, p-AKT(S473), and AKT were determined using Western blotting. β-Actin served as loading control. (b, c, e, f) Abundance of GLUT1 and GLUT4 in cytoplasm and membrane subfraction at ion was probed using Western blotting. β-Actin and Na+-K+ATPase served as loading control of cytoplasm and membrane fraction. The quantitative result (mean ± SEM) was calculated from at least three independent experiments. .