The Phenolic Components of Gastrodia elata improve Prognosis in Rats after Cerebral Ischemia/Reperfusion by Enhancing the Endogenous Antioxidant Mechanisms

Figure 7

Effects of PCGE on H₂O₂-induced LDH release from human primary astrocytes. (a) Cells were preincubated for 24 h with the different concentrations of PCGE (15, 25, and 50 μg/ml) and then exposed to 0.5 mM of H₂O₂ for 4 h in FBS-free medium. ^,Significant differences at the and levels, respectively, versus the control group; versus the 15 μg/ml group. (b) LDH release from astrocyte cells that were pretreated with 25 μg/ml PCGE for different durations (up to 48 h) before exposure to 0.5 mM H₂O₂. ^,Significant differences at the and levels, respectively, versus the control group; and versus the 1 h group. The results were quantified by using an LDH activity kit assay and expressed as percentages of LDH released in PCGE-treated or vehicle-treated cells (control) exposed to H₂O₂. Values are means ± SE; independent experiments. ANOVA followed by the Tukey post hoc test.

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