Anti-Inflammatory Effect of a Polyphenol-Enriched Fraction from Acalypha wilkesiana on Lipopolysaccharide-Stimulated RAW 264.7 Macrophages and Acetaminophen-Induced Liver Injury in Mice

Figure 4

Effect of PEF on LPS-stimulated production of proinflammatory cytokines in RAW 264.7 macrophages. RAW 264.7 macrophages were preincubated with 10–200 μg/mL PEF for 2 h and then treated with 1 μg/mL LPS for 24 h. The concentrations of (a) TNF-α, (b) IL-1β, and (c) IL-6 in the cultured medium were determined by the commercial ELISA kits. Results are shown as mean ± SD (). The different letters represent the statistical differences at among the groups by Tukey-Kramer’s test.

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