Research Article

Anti-Inflammatory Effect of a Polyphenol-Enriched Fraction from Acalypha wilkesiana on Lipopolysaccharide-Stimulated RAW 264.7 Macrophages and Acetaminophen-Induced Liver Injury in Mice

Figure 5

Effect of PEF on LPS-stimulated activation of NF-κB in RAW 264.7 macrophages. (a) RAW 264.7 macrophages were preincubated with 10–200 μg/mL PEF for 2 h and then treated with 1 μg/mL LPS for 0.5 h. The nuclear and cytoplasmic extracts of cells were prepared, and the protein level of NF-κB was determined by Western blot analysis. Lamin B and tubulin were used as endogenous controls for the nucleus and cytoplasm, respectively. (b) RAW 264.7 macrophages were preincubated with 200 μg/mL PEF for 2 h; then the total protein was harvested at different time points (15, 30, 60, and 120 min) after stimulation with LPS (1 μg/mL), and the protein levels of p-IKKα/β, IKKα, IKKβ, p-IκB-α, IκB-α, p-NF-κB, and NF-κB were determined by Western blot analysis. GAPDH was used as an endogenous control.
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