Cafestol Inhibits Cyclic-Strain-Induced Interleukin-8, Intercellular Adhesion Molecule-1, and Monocyte Chemoattractant Protein-1 Production in Vascular Endothelial Cells

Figure 1

Cafestol inhibits cyclic-strain-induced inflammatory molecule secretion in HUVECs. HUVECs grown on Flexcell plates were subjected to cyclic strain for 24 h. Control cells were left under static conditions. (a) Effect of cyclic strain (−20 kPa) applied for 24 h on the production of cytokines. Values are the (). versus static controls. (b) MCP-1 expression was detected using Western blot analysis with the corresponding antibody. GAPDH staining was used as a normalization control. The upper panels are representative of three independent experiments. Lower panel: the bar graph shows the fold increase in protein expression compared with static control cells. Soluble MCP-1 (c), ICAM-1 (d), and IL-8 (e) production in culture media was evaluated using ELISA; , versus the static control group. versus the cyclic strain group.

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