Flutamide Induces Hepatic Cell Death and Mitochondrial Dysfunction via Inhibition of Nrf2-Mediated Heme Oxygenase-1

Figure 2

Flutamide increased hydrogen peroxide (H₂O₂) accumulation and elicited mitochondrial dysfunction by a concentration-dependent manner. Cells were treated with flutamide at the concentrations up to 100 μM for 24 h. Hydrogen peroxide content was analyzed by a commercial kit based on ferrous oxidation of xylenol orange assay (a). Mitochondrial dysfunction was indicated by determination of mitochondrial membrane potential (b) and ATP content. Data are presented as the mean ± SD from 3 independent experiments. compared with the cells in the control group without drug treatment.

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