Genetically Engineered Resveratrol-Enriched Rice Inhibits Neuroinflammation in Lipopolysaccharide-Activated BV2 Microglia Via Downregulating Mitogen-Activated Protein Kinase-Nuclear Factor Kappa B Signaling Pathway

Figure 2

Treatment with resveratrol-enriched rice inhibits the expression of iNOS and COX-2 in lipopolysaccharide-activated (acute and chronic) BV2 microglial cells. BV2 microglial cells were pretreated with normal rice (NR), resveratrol-enriched rice (RR), and resveratrol after 30 min of LPS (100 ng/mL) stimulation. (a–c) iNOS and COX-2 expression and their band intensity in LPS-activated BV2 microglial cells after 6 h of sample treatment and LPS activation. (d–f) iNOS and COX-2 expression and their band intensity in LPS-activated BV2 microglial cells after 24 h of sample treatment and LPS activation. Tubulin was used as loading control. All data are presented as the mean ± standard error of the mean of three independent experiments. , , and indicate significant differences compared with LPS treatment alone. indicates significant differences compared with untreated control group. indicates significant differences to RR compared to NR. Ctl, untreated control and LPS, lipopolysaccharide.

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