Review Article

How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions

Table 2

Intracellular O2 levels under various cell culture conditions.

Cell line and conditionsExtracellular O2 (%)Intracellular O2 (%)References

Confluent, differentiated PC12 cell monolayer20~15[74]
10~7
6~1.5
HeLa20~17[75]
108-9
52–5
Undifferentiated PC12 cells20~15[76]
81-2
Mouse embryonic fibroblasts206–8[76]
Mouse embryonic fibroblasts20~14[9]
92-3
5~0.5