Review Article
How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions
Table 3
Km(O2) and Vmax values for H2O2 production from isolated liver mitochondria respiring on various substrates.
| | Substrates | Km(O2) (%) | H2O2 production (pmol min−1·mg protein−1) |
| | Glutamate + malate with malonate | 0.025 | 250 | | Succinate (− rotenone) | 0.179 | 330 | | Succinate (+ rotenone) | 0.070 | 105 | | Glutamate, malate, and succinate | 0.050 | 330 | | Palmitoylcarnitine (− rotenone) | 0.100 | 290 | | Palmitoylcarnitine (+ rotenone) | 0.398 | 250 |
| | Sites | | | | Complex III Qo site | 0.199 | 150 | | Complex I FMN site | 0.019 | 170 | | Complex I electron backflow | 0.090 | 135 | | ETFQOR | 0.498 | 200 |
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All values are from Hoffman et al. 2009 and measured using an Amplex Red/horseradish peroxidase assay in the presence of superoxide dismutase at 37°C.
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