Nrf2 Deficiency Unmasks the Significance of Nitric Oxide Synthase Activity for Cardioprotection

Table 2

Concentrations of nitrite, nitrate, nitrosation products (RXNO), and nitrosyl heme (NO heme) in blood and different tissues of Nrf2 KO mice as compared to WT mice (mean ± SD, each).

Metabolite	Concentration	WT mice	Nrf2 KO mice	value (versus WT mice)
Heart
Nitrite	μM	1.673 ± 0.4005	0.7784 ± 0.8500	↓0.0039
Nitrate	μM	57.30 ± 40.04	31.22 ± 29.14	ns
RXNO	nM	144.1 ± 26.76	177.4 ± 57.65	ns 0.0603
NO heme	nM	103.8 ± 42.02	79.40 ± 4.777	ns
Aorta
Nitrite	μM	146.1 ± 99.19	87.49 ± 15.87	ns
Nitrate	μM	2755 ± 1166	8428 ± 4852	ns
RXNO	nM	2506 ± 836.9	2712 ± 1154	ns
NO heme	nM	nd	nd	—
Liver
Nitrite	μM	0.2970 ± 0.1423	0.3772 ± 0.3052	ns
Nitrate	μM	28.63 ± 9.395	37.17 ± 9.736	ns
RXNO	nM	1040 ± 136	710.1 ± 278.8	↓0.0019
NO heme	nM	300.0 ± 67.34	162.6 ± 30.52	↓0.0032
Lung
Nitrite	μM	0.7026 ± 0.05554	0.9157 ± 0.2999	↑0.0133
Nitrate	μM	32.40 ± 3.535	38.05 ± 8.71	ns
RXNO	nM	56.56 ± 21.18	42.79 ± 24.02	ns
NO heme	nM	301.6 ± 92.45	275.0 ± 173.8	ns
Plasma
Nitrite	μM	0.9387 ± 0.1351	0.6771 ± 0.1681	↓0.0080
Nitrate	μM	27.33 ± 6.395	26.42 ± 7.61	ns
RXNO	nM	21.25 ± 2.7	8.852 ± 1.215	↓<0.0001
NO heme	nM	nd	nd	—
Erythrocytes
Nitrite	μM	1.147 ± 0.1761	0.9267 ± 0.1291	ns 0.0545
Nitrate	μM	46.87 ± 12.34	33.75 ± 13.18	ns 0.0514
RXNO	nM	177.8 ± 46.18	213.1 ± 63	ns
NO heme	nM	48.67 ± 15.46	169.0 ± 54.82	↑<0.0001

ns - not significant; ↓ - decreased; ↑ - increased; ; ; .