Oxidative Medicine and Cellular Longevity / 2018 / Article / Tab 1

Research Article

p66Shc Inactivation Modifies RNS Production, Regulates Sirt3 Activity, and Improves Mitochondrial Homeostasis, Delaying the Aging Process in Mouse Brain

Table 1

Oxidative parameters in isolated brain mitochondria of wild-type and p66Shc(−/−) aged mice.

Control (WT 3 mo)WT 24 mop66Shc(−/−) 24 mo

A. H2O2 production rate (nmoles/min/mg prot)
 1. Malate + glutamate1.66 ± 0.052.22 ± 0.1a0.97 ± 0.03b
 2. Succinate1.08 ± 0.051.35 ± 0.09a0.8 ± 0.1b
B. Superoxide anion level (AFU)101 ± 8194 ± 4.5a140 ± 14b
C. ROS level (AFU)76 ± 3111 ± 8a82 ± 4a
D. SOD activity (pmoles/min/mg prot)2824 ± 3092305 ± 153a2468 ± 94a
E. Catalase activity (units/mg prot)32 ± 529 ± 427 ± 3

Note: data are expressed as the of the different groups (). Results were contrasted in pairs between the 3-month-old WT control group and the two aged groups (a) as well as between the 24-month-old WT and p66Shc(−/−) groups (b). a and b represent , one-way analysis of variance (ANOVA) and Bonferroni post hoc test.