p66^Shc Inactivation Modifies RNS Production, Regulates Sirt3 Activity, and Improves Mitochondrial Homeostasis, Delaying the Aging Process in Mouse Brain

Table 2

Bioenergetic parameters of isolated brain mitochondria of wild-type and p66^Shc(−/−) aged mice.

	Control (WT 3 mo)	WT 24 mo	p66^Shc(−/−) 24 mo
A. Oxygen consumption rate
1. Malate + glutamate
State 3 (ng at O/min/mg protein)	102 ± 3.6	74 ± 2.4^a	125 ± 4.6^b
Respiratory control (RC)	4.3 ± 0.2	3.3 ± 0.1^a	4.4 ± 0.2
2. Succinate
State 3 (ng at O/min/mg protein)	116 ± 4.4	84 ± 3.5^a	109 ± 5^a
Respiratory control (RC)	4.1 ± 0.1	3.1 ± 0.1^a	5 ± 0.1^a
B. Respiratory chain complex activity
1. Complex I (nmol/min/mg protein)	78.4 ± 4.7	37.5 ± 3.1^a	51.8 ± 5.2^b
2. Complex II–III (nmol/min/mg protein)	98.2 ± 9.1	51.1 ± 6.7^a	48.4 ± 4.1^a
3. Complex IV (/min/mg protein)	23 ± 0.75	19.1 ± 0.45^a	15.8 ± 1^b
C. ATP synthesis rate (nmol/min/mg protein)	125 ± 1	42 ± 3^a	81 ± 2^b
D. NAD^+/NADH^+H ratio	5.3 ± 0.7	1.2 ± 0.4^a	2.7 ± 0.5^b
E. Mitochondrial potential, ∆Ψ (AFU)	601 ± 15	414 ± 25^a	520 ± 16^b

Note: data are expressed as the of the different groups (). Results were contrasted in pairs between the 3-month-old WT control group and the two aged groups (a) as well as between both the 24-month old WT and p66^Shc(−/−) groups (b). a and b represent , one-way analysis of variance (ANOVA) and Bonferroni post hoc test. AFU: arbitrary fluorescence units.