Research Article

Inhibition of Protein Aggregation by Several Antioxidants

Figure 5

Effect of resveratrol on amyloid fibril formation by stefin B. Aggregation kinetics monitored by ThT binding in the absence and presence of different concentrations of Res. The inhibitory effect on amyloid formation of stB wt was monitored by following the ThT fluorescence emission at 482 nm upon excitation at 440 nm. Concomitantly, the aggregation behavior of stB wt with final 1% v/v DMSO was followed each day as a control because Res was dissolved in DMSO to a final 1% v/v. Protein concentration was 34 μM, and concentrations of Res were varied.