Research Article
Andrographolide Ameliorates Diabetic Cardiomyopathy in Mice by Blockage of Oxidative Damage and NF-κB-Mediated Inflammation
Figure 6
Andro treatment inhibits diabetes-induced myocardial oxidative/nitrative stress. Quantification of SOD (a), MDA (b), and 4-HNE (c) levels in homogenized fresh heart tissues. (d) Immunostaining of 3-NT. (e) Myocardial O2− generation was detected by DHE staining in the freshly frozen sections. (f) Western blot analysis of iNOS levels. (g–i) Real-time qPCR analysis was performed to detect the mRNA expression of the ROS-generating NADPH oxidases (NOX2, NOX4, and p47phox) in the myocardial tissues. (j and k) The mRNA expression of Nrf2 and HO-1 in the myocardial tissues. compared to control; compared to DM.
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