Research Article

Exogenous Hydrogen Sulfide Supplement Attenuates Isoproterenol-Induced Myocardial Hypertrophy in a Sirtuin 3-Dependent Manner

Figure 1

Effect of NaHS on myocardial hypertrophy and SIRT3 expression in the mice after ISO administration. Male C57BL/6 mice at 10 weeks of age were administrated with NaHS (50 μmol/kg/d) or normal saline (NS) once daily. After 2 weeks, the mice were given isoproterenol (ISO, 60 mg/kg) by intraperitoneal injection followed by NaHS or NS administration once daily for another 2 weeks. (a–b) ANP and BNP mRNA expressions were quantified by real-time PCR. 18S was serviced as a house-keep gene. (c) Quantification of SIR2 family (SIRT1-SIRT7) mRNA expression was assessed by real-time PCR. 18S was serviced as a house-keep gene. (d) The expression of SIRT3 protein in the myocardium of mice was measured by western blot. GAPDH was serviced as a loading control. Plots represent the mean ± SEM; . Statistical significance: compared with NS; compared with ISO.
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