Exogenous Hydrogen Sulfide Supplement Attenuates Isoproterenol-Induced Myocardial Hypertrophy in a Sirtuin 3-Dependent Manner

Figure 7

Effect of NaHS on oxidative stress in WT mice and SIRT3 KO mice after ISO administration. Male 129S1/SvImJ (WT) and SIRT3 knockout (KO) mice at 10 weeks of age were administrated with NaHS (50 μmol/kg/d) or normal saline (NS) once daily. After 2 weeks, the mice were given isoproterenol (ISO, 60 mg/kg) by intraperitoneal injection followed by NaHS or NS administration once daily for another 2 weeks. (a) The myocardium of mice was stained with DHE and was photographed with a fluorescence microscope. (b) MDA level in the myocardium was measured. (c) T-AOC of the myocardium was assessed. (d) SOD, Cu-Zn/SOD, and Mn-SOD activity of myocardium were evaluated. Plots represent the mean ± SEM; . Statistical significance: compared with WT + NS; , compared with WT + ISO; compared with SIRT3 KO + NS.

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