Resveratrol-Induced Downregulation of NAF-1 Enhances the Sensitivity of Pancreatic Cancer Cells to Gemcitabine via the ROS/Nrf2 Signaling Pathways

Figure 2

The expression levels of NAF-1 and Nrf2 in four different pancreatic cancer cell lines. (a, b) The expression of NAF-1 and Nrf2 at the protein level in the Panc-1, Mia paca-2, BxPC-3, and CF pac-1 cell lines was detected by Western blotting. The quantified histograms of the Western blots were shown alongside. (c) The normalized fold expression of NAF-1 and Nrf2 at the mRNA level was estimated in four pancreatic cancer cell lines by qRT-PCR. Data are presented as the mean ± SD of three independent experiments. The bar graph shows the relative mRNA expression levels in the cell lines. Column: mean; bar: SD. (d, e) Immunofluorescence staining of NAF-1 and Nrf2 was performed to show the basic expression levels and the locations of the proteins in the cells. NAF-1 and Nrf2 staining is shown in green, and nuclear DNA staining by DAPI is shown in blue. Images are representative of three independent experiments (magnification, 400x; scale bar, 20 μm). Column: mean; bar: SD.

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