Inhibition of HDAC6 Activity Alleviates Myocardial Ischemia/Reperfusion Injury in Diabetic Rats: Potential Role of Peroxiredoxin 1 Acetylation and Redox Regulation
Effects of MI/R injury on HDAC6 activity, total Prdx1, and Ac-Prdx1 in nondiabetes and diabetic rats. Rats were subjected to 45 min of ischemia followed by 3 h of reperfusion. N: nondiabetic rats; D: STZ-induced diabetic rats; S: sham operation; IR: ischemia/reperfusion; TubA: tubastatin A. (a) HDAC6 activity was measured by fluorometric assay kit. (b) Total Prdx1 level of myocardium was analyzed by Western blot. GAPDH served as the loading control. (c) Acetylation level of Prdx1 was analyzed by immunoprecipitation. All the results are presented as mean ± SEM, /group. versus NIR group, versus NS group, and versus DS group.
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