Inhibition of HDAC6 Activity Alleviates Myocardial Ischemia/Reperfusion Injury in Diabetic Rats: Potential Role of Peroxiredoxin 1 Acetylation and Redox Regulation
Effects of TubA on ROS, mPTP, apoptosis rate, and cellular injury in H9c2 cells exposed to HG. HG: high glucose (30 mM); HR: hypoxia/reoxygenation; TubA: tubastatin A. H9c2 cardiomyocytes were subjected to 4 h of hypoxia followed by 2 h of reoxygenation with or without TubA under LG or HG stimulation. (a) Representative images of ROS staining and the mean fluorescence intensity of DCFDA in each group. (b) Representative images of flow cytometry and cardiomyocyte apoptosis rates in each group. (c) Fluorescent images of the cells show the change in integrity of mPTP. (d) Cardiomyocyte H2O2 concentration. (e) Cardiomyocyte superoxide anion production. (f) Cardiomyocyte LDH release. (g) Cardiomyocyte cell viability. All values are presented as the mean ± SEM, /group. versus HG group, versus HGHR group.
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