Oxidative Medicine and Cellular Longevity / 2018 / Article / Fig 7

Research Article

Inhibition of HDAC6 Activity Alleviates Myocardial Ischemia/Reperfusion Injury in Diabetic Rats: Potential Role of Peroxiredoxin 1 Acetylation and Redox Regulation

Figure 7

Effect of HDAC6 inhibition on HDAC6 activity, total Prdx1, and Ac-Prdx1 in H9c2 cells exposed to HG. HG: high glucose (30 mM); HR: hypoxia/reoxygenation; TubA: tubastatin A. (a) HDAC6 activity was measured by fluorometric assay kit. (b) Total Prdx1 level of H9c2 cardiomyocytes was analyzed by Western blot. GAPDH served as the loading control. (c) Acetylation level of Prdx1 was analyzed by immunoprecipitation. All values are presented as the mean ± SEM, /group. versus HG group, versus HGHR group.

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