Formulated Chinese Medicine Shaoyao Gancao Tang Reduces Tau Aggregation and Exerts Neuroprotection through Anti-Oxidation and Anti-Inflammation

Figure 5

Anti-inflammatory effects of SG-Tang and BV-2 conditioned medium preparation. (a) Experiment flow chart for LPS stimulation. BV-2 cells were seeded in 1% fetal bovine serum (FBS) and pretreated with 50 μM celecoxib (Celec.) for 8 h or 100~500 μg/ml SG-Tang (SG-T) 8~24 h followed by 1 μg/ml LPS stimulation 20 h. NO level was evaluated with Griess reagent. (b) Anti-inflammatory effect of celecoxib (Celec.) and SG-Tang (SG-T) on BV-2 cells (). and , treated vs. untreated cells. (c) Experiment flow chart for LPS/IFN-γ stimulation. For preparation of BV-2 conditioned medium (CM), BV-2 cells were seeded in Dulbecco’s modified Eagle’s medium (DMEM) with 1% FBS medium. Next day, cells were stimulated with a combination of LPS (1 μg/ml) and IFN-γ (100 ng/ml). After 24 h stimulation, the BV-2 CM was collected and examined for inflammation by morphology, Iba1 Western blotting and NO/TNF-α/IL-1β/IL-6 determination. (d) Morphology of BV-2 cells. (e) Western blot analysis of Iba1 expression in inflamed BV-2 cells (). To normalize, Iba1 expression level in uninflamed cells was set as 100%. , stimulated vs. unstimulated cells. (f) Secretion of NO, TNF-α, IL-1β, and IL-6 in BV-2 CM. and , stimulated vs. unstimulated cells.

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