Enhanced p62-NRF2 Feedback Loop due to Impaired Autophagic Flux Contributes to Arsenic-Induced Malignant Transformation of Human Keratinocytes

Figure 2

Long-term exposure to low-level arsenite induced adaptive antioxidative response in HaCaT cells. (a) Representative histogram for intracellular ROS levels detected by flow cytometer. As-TM cells or passage-matched nontreated control (Con) was challenged with 10 μM of sodium arsenite (As) or equal volume of PBS (Veh) for 24 h. (b) Quantification of intracellular ROS levels determined by flow cytometer. (c) mRNA levels of NRF2 and p62 in As-TM and control cells. (d) Western blot of NRF2 in As-TM and control cells. Upper: representative image; lower: quantification of NRF2 protein levels determined with Western blot. . , compared with Con compartment. ^#, compared with Veh compartment.

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