Amyloid Peptide β1-42 Induces Integrin αIIbβ3 Activation, Platelet Adhesion, and Thrombus Formation in a NADPH Oxidase-Dependent Manner
Effect of NOX inhibitor VAS2870 on platelet spreading on Aβ1-42. Human platelet suspension was preincubated with NOX inhibitor VAS2870 (10 μM) for 30 mins then plated on glass coverslips coated with 10 μM Aβ1-42 and 5 mg/ml BSA in PBS. The surface area of the adhered platelets fixed, permeabilized, and stained with TRITC-conjugated phalloidin is shown in (a), and representative images at 100x magnification are displayed. The quantification of the surface area of the adhered platelets (μm2/plt) is shown in (b). Statistical significance for 4 independent experiments was analysed by one-way ANOVA with Bonferroni posttest (), with bars representing standard error of the mean (SEM).
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