Amyloid Peptide β1-42 Induces Integrin αIIbβ3 Activation, Platelet Adhesion, and Thrombus Formation in a NADPH Oxidase-Dependent Manner

Figure 5

Adhesion of platelets to amyloid peptides under physiological shear stress. Flow biochips (ibidi Vena8 Fluoro+) were coated with 0.1 mg/ml fibrillary collagen or 10 μM scrambled Aβ1-42 (scAβ42), Aβ1-42, Aβ1-40, and Aβ25-35. Platelet adhesion was tested in human whole blood at shear rates of 1,000 sec⁻¹ and 200 sec⁻¹. Where indicated, 10 μM VAS2870 was added to the blood to inhibit NOXs. Pictures shown here are representative of 3 independent experiments. Surface coverage analysis was performed using ImageJ and statistically analysed by one-way ANOVA with Bonferroni posttest ().

(a) Collagen—1000 sec⁻¹

(b) Aβ42—1000 sec⁻¹

(c) Aβ42—200 sec⁻¹

(d) —200 sec⁻¹

(e) Aβ40—200 sec⁻¹

(f) scAβ42—200 sec⁻¹

(g) Platelet adhesion at 200 sec⁻¹