Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 5

Research Article

Amyloid Peptide β1-42 Induces Integrin αIIbβ3 Activation, Platelet Adhesion, and Thrombus Formation in a NADPH Oxidase-Dependent Manner

Figure 5

Adhesion of platelets to amyloid peptides under physiological shear stress. Flow biochips (ibidi Vena8 Fluoro+) were coated with 0.1 mg/ml fibrillary collagen or 10 μM scrambled Aβ1-42 (scAβ42), Aβ1-42, Aβ1-40, and Aβ25-35. Platelet adhesion was tested in human whole blood at shear rates of 1,000 sec−1 and 200 sec−1. Where indicated, 10 μM VAS2870 was added to the blood to inhibit NOXs. Pictures shown here are representative of 3 independent experiments. Surface coverage analysis was performed using ImageJ and statistically analysed by one-way ANOVA with Bonferroni posttest ().
(a) Collagen—1000 sec−1
(b) Aβ42—1000 sec−1
(c) Aβ42—200 sec−1
(d) —200 sec−1
(e) Aβ40—200 sec−1
(f) scAβ42—200 sec−1
(g) Platelet adhesion at 200 sec−1

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.