Research Article

Pterostilbene Attenuates Fructose-Induced Myocardial Fibrosis by Inhibiting ROS-Driven Pitx2c/miR-15b Pathway

Figure 8

Pterostilbene and allopurinol reduce fructose-induced cardiac ROS to block Pitx2c-mediated miR-15b low expression in H9c2 cells. Cellular ROS production (a), NADPH oxidase activity (b), Pitx2c protein (c), and miR-15b expression (d) were determined in NAC-pretreated H9c2 cells coincubated with 5 mM fructose, 10 μM pterostilbene, and 30 μM allopurinol (), respectively. Cellular NADPH oxidase activity and ROS production were determined in miR-15b mimic (e, f) or miR-15b inhibitor (g, h)-transfected H9c2 cells (). Relative protein levels of Pitx2c were normalized to β-actin. The relative miR-15b expression levels were normalized to U6. Data are expressed as the #, ##, and ### vs. normal cell control group; , , and vs. fructose-vehicle cell group or fructose-vehicle+NAC or miR-15b mimic or miR-15b inhibitor control cell group.
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