Oxidized Cell-Free DNA Role in the Antioxidant Defense Mechanisms under Stress

Figure 3

(a) Staining of primary cell culture of Wistar rat cerebellum with 8-oxodG-antibody (PE). From left to right: control and oxyDNA-treated cells (180 min). Nuclei are stained with DAPI. ×40. (b) Staining of primary cell culture of Wistar rat cerebellum with NRF2-antibody (FITC). From left to right: control and oxyDNA-treated cells (180 min). Nuclei are stained with DAPI. ×40. (с) Oxidized cfDNA (50 ng/mL) effect on Nrf2 and Hmox1 expression in the mixed culture of neurons and glia. The incubation time is shown on the graph. Expression was detected by RT-PCR relatively to housekeeping gene TBP. vs. the control group.

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