Targeting IL-17A Improves the Dysmotility of the Small Intestine and Alleviates the Injury of the Interstitial Cells of Cajal during Sepsis
Histological analyses of the small intestine and changes in ICCs in normal mice, septic mice treated with PBS, and septic mice treated with IL-17A-Ab. (a) Small intestine samples were obtained 48 h after CLP. The histologic scoring of hematoxylin and eosin (H&E) staining was assessed by intestinal injury grading. Scale bar: 0.2 mm. (b) Immunohistochemical staining for c-Kit was performed to analyze ICC networks. The immunofluorescence intensity of c-Kit was assessed. Scale bar: 50 μm. (c) RT-PCR analysis of the c-Kit and Ano-1 expression in the small intestinal muscularis propria. (d) Ultrastructural changes in ICC morphology were examined by TEM analysis (10,000x magnification). The damage to ICCs (white) and swelling of the nerve filament (black) are indicated by arrows (data are shown as the , to 7, ).
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