Protective Effects of Inorganic and Organic Selenium on Heat Stress in Bovine Mammary Epithelial Cells

Figure 4

Selenite methionine (SM) and sodium selenite (SS) improved redox status in high temperature-shocked MAC-T cells. MAC-T cells were pretreated with (+) or without (-) 10 μM SM or 1 μM SS for 2 h, followed by 42.5°C treatment for 1 h (heat treatment, HT) and recovery at 37°C for 12 h. The cells were analyzed for (a) the intracellular reactive oxygen species (ROS) production; (b) relative mRNA abundance of HO-1; (c) the superoxide dismutase (SOD) activity and total antioxidant capacity (T-AOC); (d) relative mRNA abundance of nuclear factor erythroid 2-related factor 2 (Nrf2) and its target gene thioredoxin reductase 1 (TXNRD1); and (e) protein levels of Nrf2 and TXNRD1 (top panel: a representative western blot image, bottom panel: quantitative representation of the western blot analysis of 3 independent experiments). Values without a common letter are different ().

(a)

(b)

(c)

(d)

(e)