Research Article

5-Azacytidine and Resveratrol Enhance Chondrogenic Differentiation of Metabolic Syndrome-Derived Mesenchymal Stem Cells by Modulating Autophagy

Figure 1

Immunophenotyping and multipotency assay. Expression of CD44, CD90, and CD 45 was investigated with flow cytometry (a). Effectiveness of differentiation was established by specific staining (b). Safranin stained proteoglycans formed during chondrogenic differentiation while Alizarin Red stained extracellular mineralized matrix formed in the course of osteogenic differentiation. Intracellular lipid droplets formed during adipogenesis were stained with Oil Red dye. Scale bar: 500 μM.
(a)
(b)