Cytotoxic Effects of Artemisia annua L. and Pure Artemisinin on the D-17 Canine Osteosarcoma Cell Line

Figure 2

Representative image of cell cycle analysis (a) and nuclear staining with Hoechst 33342 (b) of D-17 cells after 24 hours of treatment: (A) untreated cells and in the presence of (B) DMSO, (C) 548 μM artemisinin, (D) a solution of 65% ethanol, and (E) hydroalcoholic extract corresponding to 65 μM artemisinin. (c) Percentage of apoptotic nuclei. (d) Cell cycle distribution. Data are reported as the (). Significant differences vs. untreated cells, comparing cell cycle phases (G₀/G₁, S, G₂/M, and Sub-G₁), are indicated by and by and (two-way ANOVA followed by the Bonferroni multiple comparisons). Arrows indicate apoptotic nuclei. .

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