[Retracted] The Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneas

Figure 6

The immunohistochemical expression of VEGF and corneal neovascularization in UVB-irradiated corneas of rabbits treated with trehalose (3% or 6% concentration) drops or buffer drops for fourteen days after the end of repeated irradiation. The expression of VEGF is absent in control corneas (a) and nearly absent in UVB-irradiated corneas treated with drops of 6% trehalose (c). Slight expression of VEGF is seen in corneas treated with 3% trehalose (arrow) (b). In contrast, after the treatment of UVB-irradiated corneas with buffer drops, VEGF is highly expressed in corneas (arrow) (d). Scale bars: 50 μm. The quantification of VEGF genes by real-time PCR is shown in (e). Each bar represents the from 6 corneas. The values with asterisks are significantly different (, ) from those of UVB-irradiated buffer-treated corneas. Quantification of corneal neovascularization (number of vessels) is summarized in (f). Trehalose treatment highly suppressed corneal neovascularization. Each bar represents the from 6 corneas. The values with asterisks are significantly different () from those of buffer-treated UVB-irradiated corneas.