Verapamil Attenuated Prediabetic Neuropathy in High-Fat Diet-Fed Mice through Inhibiting TXNIP-Mediated Apoptosis and Inflammation

Figure 5

The effect of TXNIP inhibition on cell apoptosis and promoted cell viability in palmitate-treated neurons. (a) The changes in the Δψm based on JC-1 staining were measured by a flow cytometer. The intensity of green fluorescence delegates JC-1 monomer which is quantified in FL-1 (-axis), while the intensity of red fluorescence delegates J-aggregates which is quantified in FL-2 (-axis). The proportions of green fluorescence and red fluorescence were displayed on the right. The experiments were performed in triplicate. (b) The activity of caspase-3 was measured by pNA, was released from the caspase substrate Ac-DEVD-pNA, and was reported as OD 405 nM/mg protein. (c) The cell viability was measured by MTT assay. The experiments were performed in triplicate, and each value represents mean ± SD. ; ; .

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