Research Article

CIAPIN1 Targeted NHE1 and ERK1/2 to Suppress NSCLC Cells’ Metastasis and Predicted Good Prognosis in NSCLC Patients Receiving Pulmonectomy

Figure 3

Validation of CIAPIN1 overexpression was assessed in A549 cells. (a) The representative photographs of 293T cells and A549 cells with GFP expression were shown. (b) The immunofluorescence assay was performed to detect the increase in CIAPIN1 observed with a confocal laser microscope. Blue indicated nuclei, green indicated GFP, and red indicated CIAPIN1 staining. (c) The mRNA and protein expressions of CIAPIN1 in A549 cells with CIAPIN1 overexpression were detected by real-time quantitative PCR and western blotting. Each value indicated the of three independent experiments. , compared with control.
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