Berberine Ameliorates Doxorubicin-Induced Cardiotoxicity via a SIRT1/p66Shc-Mediated Pathway
si-SIRT1 attenuated the protective effect of Ber in DOX-induced injured cardiomyocytes. H9c2 cardiomyocytes were exposed to control siRNA or SIRT1 siRNA and then treated with Ber (1 μmol/L for 8 h). Then, the cells were exposed to 1 μmol/L DOX for a 4 h treatment. Then, SIRT1-related signalling and oxidation-related proteins were measured. (a) Effects of Ber and SIRT1 siRNA on SIRT1 and p66Shc expression: (A) representative blots of p66Shc expression; (B) representative blots of SIRT1 expression. (b) si-SIRT1 restricted the antioxidative activity of Ber in DOX-stimulated H9c2 cells: (A) cardiac LDH level in rats; (B) cardiac SOD level in rats; (C) cardiac MDA level in rats. The results are expressed as the , /group. △△ vs. control siRNA group, ## vs. DOX+control siRNA group, and or vs. DOX+Ber+control siRNA group.
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