Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 6

Research Article

Berberine Ameliorates Doxorubicin-Induced Cardiotoxicity via a SIRT1/p66Shc-Mediated Pathway

Figure 6

si-SIRT1 attenuated the protective effect of Ber in DOX-induced injured cardiomyocytes. H9c2 cardiomyocytes were exposed to control siRNA or SIRT1 siRNA and then treated with Ber (1 μmol/L for 8 h). Then, the cells were exposed to 1 μmol/L DOX for a 4 h treatment. Then, SIRT1-related signalling and oxidation-related proteins were measured. (a) Effects of Ber and SIRT1 siRNA on SIRT1 and p66Shc expression: (A) representative blots of p66Shc expression; (B) representative blots of SIRT1 expression. (b) si-SIRT1 restricted the antioxidative activity of Ber in DOX-stimulated H9c2 cells: (A) cardiac LDH level in rats; (B) cardiac SOD level in rats; (C) cardiac MDA level in rats. The results are expressed as the , /group. △△ vs. control siRNA group, ## vs. DOX+control siRNA group, and or vs. DOX+Ber+control siRNA group.

We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.