Increased Transfection of the Easily Oxidizable GC-Rich DNA Fragments into the MCF7 Breast Cancer Cell

Figure 7

Expression of EGFP in MCF7 treated with pEGFP-rDNA or pEGFP for 24 h. (a) qPCR. Quantitation of pEGFP and pEGFP-rDNA in the cellular DNA after 3, 24, and 72 hours of incubation. pEGFP content (3 h) is taken as one unit. (b) RT-qPCR. The levels of EGFP-encoding RNAs in the cells exposed to pEGFP or pEGFP-rDNA. (c) FCA. (1)—nonfixed cell plots: SSC versus FCS; R—the analyzed cell subpopulation. Distribution of FL1-EGFP fluorescence intensities. R1- and R2-gated areas. (2)—median signal intensity of FL1-EGFP (gate R1). (3)—relative fractions of EGFP-positive cells (R2 gate). (d) FM-based evaluation of EGFP in the cells treated with pEGFP-rDNA or pEGFP-rDNA/Turbo Fect (×40).

(a)

(b)

(c)

(d)