Antagonizing Effects of Clematis apiifolia DC. Extract against Benzo[a]pyrene-Induced Damage to Human Keratinocytes

Figure 4

The CAE-induced activation of ARE signaling was attenuated by knockdown of Nrf2. (a) HaCaT cells were transfected with the ARE-Luc reporter and siRNA for the Nrf2 gene using the DharmaFECT® Duo transfection reagent. After 24 h, the cells were incubated with CAE (100 μg/ml) for 14 h and then subjected to the luciferase reporter assay. vs. CAE-treated control. The results were confirmed by repeating three independent experiments. Data are expressed as the (b, c) HaCaT cells were transfected with siRNA for the Nrf2 gene using the DharmaFECT® Duo transfection reagent. After 24 h, the cells were incubated with CAE (100 μg/ml) for 14 h. The cells were then subjected to Western blot (b, d) and real-time PCR (C) analyses for Nrf2, NQO1, and HO-1. vs. untreated control; ^o vs. CAE-treated control. The results were confirmed by repeating three independent experiments. Data are expressed as the

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