Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 5

Research Article

Antagonizing Effects of Clematis apiifolia DC. Extract against Benzo[a]pyrene-Induced Damage to Human Keratinocytes

Figure 5

CAE-induced activation of NRF2/HO1 pathway was not linked with AHR activation. (a, b) HaCaT cells were transfected with control siRNA, AhR siRNA, or NrF2 siRNA using the DharmaFECT® Duo transfection reagent. After 24 h, the cells were incubated with CAE (100 μg/ml) in the presence of B[a]P for 24 h and then subjected to real-time PCR analysis for HO-1 and CYP1A1. vs. CAE-treated control (a). vs. B[a]P-treated control (b). o vs. B[a]P+CAE-treated control. The results were verified by repeating three independent experiments. Data are expressed as the