Research Article

Interactions between Cytosolic Phospholipase A2 Activation and Mitochondrial Reactive Oxygen Species Production in the Development of Ventilator-Induced Diaphragm Dysfunction

Figure 1

Interactions between cPLA2 and mitochondrial ROS generation in the diaphragm following MV. (a) Western blots for cPLA2 and p-cPLA2 in the diaphragm; (b) cPLA2 activity assay; (c) mitochondrial H2O2 generation in the diaphragm; (d) correlation analysis between cPLA2 activities and mitochondrial H2O2 levels. MV = mechanical ventilation; cPLA2 = cytosolic phospholipase A2; CDIBA = 4-{2-[5-chloro-1-(diphenyl-methyl)-2-methyl-1H-indol-3-yl]-ethoxy} benzoic acid, a specific cPLA2 inhibitor; MitoTEMPO = a mitochondria-targeted antioxidant. and vs. the control group; and vs. the MV group.