Interactions between Cytosolic Phospholipase A2 Activation and Mitochondrial Reactive Oxygen Species Production in the Development of Ventilator-Induced Diaphragm Dysfunction
Hypoxia induced cPLA2 activation and mitochondrial ROS generation in vitro. (a) Western blots for HIF-1α expressions in C2C12 cells; (b) changes of cPLA2 activities in C2C12 cells underwent indicated time of hypoxia; (c) effects of AACOCF3 and SS31 on cPLA2 activities in C2C12 cells after 12 hours of hypoxia; (d) MitoSOX assay for the detection of mitochondrial ROS generation in C2C12 cells; (e) fluorescence intensities of MitoSOX; (f) effects of AACOCF3 and SS31 on calpain activities in C2C12 cells after 12 hours of hypoxia. AACOCF, a specific inhibitor of cPLA2; SS31, a mitochondria-targeted antioxidant. and vs. the control group; and vs. the MV group.