Ventilation-Like Mechanical Strain Modulates the Inflammatory Response of BEAS2B Epithelial Cells

Figure 3

Effect of cyclic stretch on nuclear NF-κB translocation. (a) Representative images of BEAS2B nonstimulated control cells (), cells subjected to sinusoidal (), rectangular (), or triangular () cycling strain for 4 h. Left: nuclei visualized with DAPI (blue); right: NF-κB distribution (green). Arrowheads indicate nuclei with elevated NF-κB content. The scaling bar represents 20 μm. (b) Densitometric evaluation of the ratio of total (green contours) to nuclear (red contours) NF-κB signal. Box plots indicate median, upper/lower quartile, and bars with minimal/maximal values. Statistics: analysis of variance (Tukey’s post hoc test). vs. control.

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