Protective Effect of Dinitrosyl Iron Complexes with Glutathione in Red Blood Cell Lysis Induced by Hypochlorous Acid

Figure 3

Effect of DNIC on hemolysis in HOCl production by myeloperoxidase (MPO). The characteristic kinetic curves of hemolysis induced by the addition of 50 nM MPO and 100 μM Н₂О₂ to RBC suspensions in the absence and in the presence of 0.5 and 2.5 μM DNIC with glutathione (a). In the presence of only MPO (blue) or hydrogen peroxide (red), the lysis of red blood cells does not occur (b).

(a)

(b)