Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 6

Research Article

Protective Effect of Dinitrosyl Iron Complexes with Glutathione in Red Blood Cell Lysis Induced by Hypochlorous Acid

Figure 6

The EPR spectra of BSA-DNIC in the presence and in the absence of HOCl. Both types of albumin-bound DNIC (DNIC-BSA-Cys and DNIC-BSA-GS) were obtained by adding cysteine (DNIC-Cys) and glutathione (DNIC-GS) to bovine serum albumin (BSA), respectively. The EPR spectrum of DNIC-BSA-Cys (a). The EPR spectrum recorded after the addition of 4 mM HOCl to 0.9 mM DNIC-BSA-Cys (b). The EPR spectrum was recorded in the DNIC-GS+DNIC-BSA-GS mixture (c); the same after addition of 4 mM HOCl (d).

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