Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid

Figure 1

Effects of 15s-HpETE on the redox state of recombinant PTEN. Recombinant PTEN was prereduced with 1 mM DTT for 120 min, followed by passing through a NAP-5 column to remove excess DTT and exposure to various concentrations of 15s-HpETE for 30 min (a) or 5 μM 15s-HpETE for the indicated times (b). The reaction was quenched by 2 mM NEM to block free thiols for 10 min. The samples were then treated with or without 1 mM DTT for reduction and subjected to nonreducing SDS-PAGE, followed by immunoblotting for PTEN.

(a)

(b)