Modulation of Antioxidant Potential with Coenzyme Q10 Suppressed Invasion of Temozolomide-Resistant Rat Glioma In Vitro and In Vivo

Figure 4

Effects of single and combined CoQ10 and TMZ treatments on gene and protein expression of antioxidant enzymes. Real-time qRT-PCR and Western blot analysis were used to assess (a) MnSOD, (b) CuZnSOD, (c) CAT, (d) GR, and (e) iNOS mRNA and protein expression after 24 h treatment with 10 μM CoQ10, 250 μM TMZ, or their combination. The mRNA expression and protein expression were normalized to the internal control, β-actin and GAPDH, respectively. Relative protein expression is accompanied by representative immunoblots. The average ±SEM was obtained from at least three independent experiments. Statistical significance is presented as (), (), and ().

(a)

(b)

(c)

(d)

(e)