Carvacrol and Thymol Modulate the Cross-Talk between TNF-α and IGF-1 Signaling in Radiotherapy-Induced Ovarian Failure

Figure 3

Immunohistochemical localization of PCNA in ovarian follicles was studied 4 days after irradiation. (a) Expression of PCNA in ovaries of the control group showed a high degree of PCNA expression in the oocyte and granulosa cells of growing follicles (brown color). (b) Expression of PCNA in the ovaries of rats subjected to γ-radiation (3.2 Gy) showed a decreased expression with O and GC. (c and d) Expression of PCNA in the ovaries of rats treated with carvacrol or thymol and exposed to γ-radiation (c) or showed a high PCNA expression of oocyte and granulosa cells of all growing follicles (brown color) (d). Scale bar, 20 μm. GC: granulosa cells, O: oocyte.

(a)

(b)

(c)

(d)